Lab 10

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Lab 10 Objectives

Identification of Unknown Bacteria (continued): 

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Background and Purpose:  

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Period 2:

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Evaluation of Cultural Characteristics:

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Nutrient broth cultures

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Evaluation of Physiological Tests:

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Gelatin Liquefaction Test

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Oxygen Requirements Test

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Evaluation of Morphological Characteristics:
bulletMotility Determination (see LAB 6)
bulletCapsule Stain (see LAB 4)

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New Inoculations: 1-nutrient agar slant tube, 1-Tryptone broth tube, 1-MR-VP broth tube, 1-Simmon's citrate agar slant tube

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Period 3: 

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Evaluation of Physiological Tests:
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Gelatin Liquefaction Test (re-evaluate nutrient gelatin culture if it was negative during Period 2)

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The IMViC Tests
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Indole Test

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Methyl Red Test

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Voges-Proskauer Test

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Citrate Utilization Test

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Evaluation of Morphological Characteristics:

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Spore Stain (see LAB 5)

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Acid-Fast Stain (see LAB 6)

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New Inoculations: 1-phenol red glucose broth tube, 1-phenol red lactose broth tube, 1-phenol red sucrose broth tube, 1-phenol red mannitol broth tube, 1-nitrate broth tube, 1-urea broth tube, 1-phenylalanine agar slant tube, 1-Kligler iron agar deep tube, 1-tryptic soy agar slant tube, 1-starch agar plate, 1-skim milk agar plate, 1-spirit blue agar plate

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This page was last modified November 25, 2008
milostam@alpenacc.edu